In partnership with the Association of Washington Cities (AWC) and the Washington State Department of Transportation’s Highways and Local Programs (HLP), the Washington Department of Fish and Wildlife (WDFW) inventoried, assessed, and prioritized fish passage barriers in anadromous waters associated with city streets in the Puget Sound region. The Puget Sound region is defined as watersheds that drain into the Puget Sound, Hood Canal, and Strait of Juan de Fuca. The project also included a reassessment and update of available city inventory data. The inventories and associated habitat surveys were conducted utilizing methods described in WDFW’s Fish Passage Barrier and Surface Water Diversion Screening Assessment and Prioritization Manual (WDFW 2009). This final report summarizes the results of the Puget Sound Cities Fish Passage Inventory work completed between June 1, 2012 and June 30, 2015. Detailed Fish Passage and Diversion Screening Inventory (FPDSI) database reports and Priority Index (PI) values will be provided directly to each city in Table 1.